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APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

《农业科学与工程前沿（英文）》 2021年 第8卷 第4期   页码 662-662 doi: 10.15302/J-FASE-2021408

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Degradation of SARS-CoV-2 receptor ACE2 by the E3 ubiquitin ligase Skp2 in lung epithelial cells

Guizhen Wang, Qun Zhao, Hui Zhang, Fan Liang, Chen Zhang, Jun Wang, Zhenyin Chen, Ran Wu, Hong Yu, Beibei Sun, Hua Guo, Ruie Feng, Kaifeng Xu, Guangbiao Zhou

《医学前沿（英文）》 2021年 第15卷 第2期   页码 252-263 doi: 10.1007/s11684-021-0837-6

摘要： An unexpected observation among the COVID-19 pandemic is that smokers constituted only 1.4%−18.5% of hospitalized adults, calling for an urgent investigation to determine the role of smoking in SARS-CoV-2 infection. Here, we show that cigarette smoke extract (CSE) and carcinogen benzo(a)pyrene (BaP) increase mRNA but trigger ACE2 protein catabolism. BaP induces an aryl hydrocarbon receptor (AhR)-dependent upregulation of the ubiquitin E3 ligase Skp2 for ACE2 ubiquitination. ACE2 in lung tissues of non-smokers is higher than in smokers, consistent with the findings that tobacco carcinogens downregulate ACE2 in mice. Tobacco carcinogens inhibit SARS-CoV-2 spike protein pseudovirions infection of the cells. Given that tobacco smoke accounts for 8 million deaths including 2.1 million cancer deaths annually and Skp2 is an oncoprotein, tobacco use should not be recommended and cessation plan should be prepared for smokers in COVID-19 pandemic.

关键词： SARS-CoV-2     tobacco smoke     benzo(a)pyrene     ACE2     Skp2    

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APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

《农业科学与工程前沿（英文）》 2021年 第8卷 第2期

摘要：

Drought stress typically causes heavy losses in apple production and uncovering the mechanisms by which apple tolerates drought stress is important in apple breeding. MdSIZ1 is a SUMO (small ubiquitin-like modifier) E3 ligase that promotes SUMO binding to substrate proteins. Here, we demonstrate that MdSIZ1 in apple has a negative relationship with drought tolerance. MdSIZ1 RNAi transgenic apple trees had a higher survival rate after drought stress. During drought stress they had higher leaf water potential, reduced ion leakage, lower H2O2 and malondialdehyde contents, and higher catalase activity. In addition, MdSIZ1 RNAi transgenic plants had a higher net photosynthetic rate during the latter period of drought stress. Finally, the transgenic apple trees also altered expression levels of some microRNAs in response to drought stress. Taken together, these results indicate that apple MdSIZ1 negatively regulates drought stress by enhancing leaf water-holding capacity and antioxidant enzyme activity.

 

关键词： apple / drought tolerance / gene expression / MdSIZ1    

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Identification of an E3 ligase-encoding gene

Yanfei Zhang, Xinchun Zhao, Yongchun Zhou, Min Wang, Guangbiao Zhou

《医学前沿（英文）》 2020年 第14卷 第3期   页码 318-326 doi: 10.1007/s11684-019-0708-6

摘要： In order to unveil ubiquitin pathway genes (UPGs) that are essential for non-small cell lung cancer (NSCLC) cell proliferation, we recently conducted a siRNA screening experiment to knockdown the expression of 696 UPGs found in the human genome in A549 and H1975 NSCLC cells. We found that silencing of one of the candidates, that encodes an E3 ubiquitin ligase essential for the repair of DNA interstrand cross-links in response to DNA damage, led to dramatic inhibition of NSCLC cell proliferation with significant -scores. Knockdown of suppressed colony forming activity of NSCLC cells. We further evaluated the significance of in NSCLCs and found that this gene was more elevated in tumor samples than in paired normal lung tissues and was inversely associated with the clinical outcome of patients with NSCLC. Moreover, expression was significantly higher in smokers than in non-smokers. These results show for the first time that is required for NSCLC cell proliferation and may have an important role in lung carcinogenesis.

关键词： RFWD3     NSCLC     prognosis     tobacco smoke    

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mTOR-targeted cancer therapy: great target but disappointing clinical outcomes, why?

Shi-Yong Sun

《医学前沿（英文）》 2021年 第15卷 第2期   页码 221-231 doi: 10.1007/s11684-020-0812-7

摘要： The mammalian target of rapamycin (mTOR) critically regulates several essential biological functions, such as cell growth, metabolism, survival, and immune response by forming two important complexes, namely, mTOR complex 1 (mTORC1) and complex 2 (mTORC2). mTOR signaling is often dysregulated in cancers and has been considered an attractive cancer therapeutic target. Great efforts have been made to develop efficacious mTOR inhibitors, particularly mTOR kinase inhibitors, which suppress mTORC1 and mTORC2; however, major success has not been achieved. With the strong scientific rationale, the intriguing question is why cancers are insensitive or not responsive to mTOR-targeted cancer therapy in clinics. Beyond early findings on induced activation of PI3K/Akt, MEK/ERK, and Mnk/eIF4E survival signaling pathways that compromise the efficacy of rapalog-based cancer therapy, recent findings on the essential role of GSK3 in mediating cancer cell response to mTOR inhibitors and mTORC1 inhibition-induced upregulation of PD-L1 in cancer cells may provide some explanations. These new findings may also offer us the opportunity to rationally utilize mTOR inhibitors in cancer therapy. Further elucidation of the biology of complicated mTOR networks may bring us the hope to develop effective therapeutic strategies with mTOR inhibitors against cancer.

关键词： mTOR     cancer therapy     resistance     GSK3     protein degradation     E3 ubiquitin ligase     PD-L1    

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APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

Baohua CHU, Jia SUN, Huan DANG, Ziqing MA, Shuang ZHAO, Qingmei GUAN, Xuewei LI

《农业科学与工程前沿（英文）》   页码 247-261 doi: 10.15302/J-FASE-2021388

摘要： Drought stress typically causes heavy losses in apple production and uncovering the mechanisms by which apple tolerates drought stress is important in apple breeding. MdSIZ1 is a SUMO (small ubiquitin-like modifier) E3 ligase that promotes SUMO binding to substrate proteins. Here, we demonstrate that in apple has a negative relationship with drought tolerance. RNAi transgenic apple trees had a higher survival rate after drought stress. During drought stress they had higher leaf water potential, reduced ion leakage, lower H O and malondialdehyde contents, and higher catalase activity. In addition, RNAi transgenic plants had a higher net photosynthetic rate during the latter period of drought stress. Finally, the transgenic apple trees also altered expression levels of some microRNAs in response to drought stress. Taken together, these results indicate that apple MdSIZ1 negatively regulates drought stress by enhancing leaf water-holding capacity and antioxidant enzyme activity.

关键词： apple     drought tolerance     gene expression     MdSIZ1    

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Regulation of NLR stability in plant immunity

Tao WANG, Jiaxin LI, Qian-Hua SHEN

《农业科学与工程前沿（英文）》 2019年 第6卷 第2期   页码 97-104 doi: 10.15302/J-FASE-2018248

摘要：

Plant nucleotide binding domain and leucine-rich repeat (NLR) receptors recognize pathogen effectors directly or indirectly and mediate innate immune responses. NLR-mediated immunity also has direct impacts on plant growth and development, as well as yield and survival. The levels of NLR proteins are therefore intricately controlled in plants to balance defense responses and other processes. In recent years, the ubiquitination-26S proteasome system and the HSP90 chaperones have emerged as having key functions in the regulation of NLR stability. The N-end rule pathway of protein degradation is also directly linked to NLR stability. Recent progress in the regulation of NLR stability and turnover is summarized here, focusing on the key components and pathways.

关键词： E3 ubiquitin ligase     degradation     nucleotide-binding leucine-rich repeat receptor     plant immunity     proteasome     protein stability     ubiquitination    

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2020第四届环境与能源工程国际会议(IC3E 2020)

会议日期： 2020年03月12日

会议地点： 海南海口 三亚

主办单位： APISE

基于S3C2410的GPRS数据终端的设计

孙学用

《中国工程科学》 2007年 第9卷 第12期   页码 94-96

摘要： 提出了以S3C2410处理器为核心，以SIM100-E为GPRS通信模块,构建了一套嵌入式Internet数据终端的方案，对其硬件构成及工作流程进行了讨论，描述了其软件层次结构，并详细讲解了基于该平台的

关键词： GPRS；嵌入式Internet；S3C2410；SIM100-E    

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一种高效的异构车辆集群协同框架 Research Articles

Iftikhar AHMAD1,2,Rafidah Md NOOR2,Zaheed AHMED3,Umm-e-HABIBA3,Naveed AKRAM4, 5,Fausto Pedro GARCÍA MÁRQUEZ6

《信息与电子工程前沿（英文）》 2021年 第22卷 第9期   页码 1247-1259 doi: 10.1631/FITEE.2000260

摘要： 异构车辆集群集成了多种类型通信网络，可在各种车辆应用中高效运行。长期演进（LTE）和专用短程通信的集成是异构网络的一种普遍形式。这种网络基础设施的异构性和成本/数据共享的不合作性是需要解决的潜在问题。车辆集群框架是解决这些问题的一种方案，但是该框架在部署到现实世界前应经过形式验证。为解决这些问题，提出一种异构的车辆集群框架，称为目标和兴趣感知集群框架。该框架集成了车载自组织网络与LTE网络，以提高道路交通效率。在此基础上，给出该框架的模型系统。使用模型检测技术对该模型进行形式验证，在功能层面评估其性能。为在微观层面评估该框架性能，通过整合最先进的工具创建异构仿真环境。仿真结果表明，与其他方法相比，该框架具有更好性能。

关键词： 车辆集群；异构性；协同；形式验证；系统模型    

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Genetic variants in the ADD1 and GNB3 genes and blood pressure response to potassium supplementation

Collaborative Research Group, De-Pei LIU PhD, Lai-Yuan WANG PhD, Jing CHEN MD, MSc, Jiang HE MD, PhD, Cashell E.RAO PhD, Charles GU PhD, James E. HIXSON PhD, Chung-Shiuan CHEN MS8, Paul K.

《医学前沿（英文）》 2010年 第4卷 第1期   页码 59-66 doi: 10.1007/s11684-010-0015-8

摘要： Dietary potassium-supplementation has been associated with a decreased risk of hypertension and other cardiovascular outcomes. However, blood pressure (BP) responses to potassium supplementation vary among individuals. This study was designed to examine the association between 12 single nucleotide polymorphisms (SNPs) in the adducin 1 alpha (ADD1) and guanine nucleotide binding protein (G protein) beta polypeptide 3 (GNB3) genes and systolic BP (SBP), diastolic BP (DBP), and mean arterial pressure (MAP) responses to potassium-supplementation. We conducted a 7-day high-sodium intervention (307.8 mmol sodium/day) followed by a 7-day high-sodium with potassium-supplementation (60 mmol potassium/day) among 1906 Han Chinese participants from rural north China. BP measurements were obtained at the end of each intervention period using a random-zero sphygmomanometer. We identified significant associations between ADD1 variant rs17833172 and SBP, DBP, and MAP responses to potassium-supplementation (all <0.0001) that remained significant after adjustment for multiple comparisons. In participants that were heterozygous or homozygous for the G allele of this marker, SBP, DBP, and MAP response to potassium-supplementation were −3.52 (−3.82, −3.21), −1.41 (−1.66, −1.15) and −2.12 (−2.37, −1.87), respectively, as compared to the corresponding responses of 1.99 (0.25, 3.73), −0.65 (−0.10, −0.21), and −0.23 (−0.37, 0.83), respectively, for those who were homozygous for A allele. In addition, participants with at least one copy of the G allele of rs12503220 of the ADD1 gene had significantly increased DBP and MAP response to potassium-supplementation ( = 0.0041 and 0.01, respectively), which was also significant after correction for multiple testing. DBP and MAP responses to potassium-supplementation were −1.36 (−1.63, −1.10) and −2.07 (−2.32, −1.82) for those with at least G allele compared to corresponding responses of 0.86 (−0.68, 2.40) and −0.45 (−1.74, 0.84) for those who were homozygous for A allele. In summary, our study identified novel associations between genetic variants of the ADD1 gene and BP response to potassium-supplementation, which could have important clinical and public health implications. Future studies aimed at replicating these novel findings are warranted.

关键词： blood pressure     genetics     polymorphism     die-tary potassium     potassium sensitivity     adducin 1 alpha (ADD1)     guanine nucleotide binding protein beta polypeptide 3 (GNB3)    

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基于过滤—群聚注意力的低光增强递归网络 Research Article

Fatma KHALLAF1,2, Walid EL-SHAFAI1,3, El-Sayed M. EL-RABAIE1, Naglaa F. SOLIMAN4, Fathi E.

《信息与电子工程前沿（英文）》 2023年 第24卷 第7期   页码 1045-1061 doi: 10.1631/FITEE.2200372

摘要： 本文提出一种基于离散四元数分数阶傅里叶变换（DQFrFT）水印和三维混沌逻辑映射（3D-CLM）加密的彩色医学图像多级隐私系统。其次，采用3D-CLM加密提高系统安全性和效率，使其可以作为多级隐私系统。仿真结果表明，所提出的安全系统能有效抵御多种类型的信道噪声攻击。

关键词： 彩色医学图像；四元数；自适应水印；加密；分数阶变换；三维混沌逻辑映射（3D-CLM）    

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The function and regulation of OTU deubiquitinases

Jiansen Du, Lin Fu, Yingli Sui, Lingqiang Zhang

《医学前沿（英文）》 2020年 第14卷 第5期   页码 542-563 doi: 10.1007/s11684-019-0734-4

摘要： Post-translational modification of cellular proteins by ubiquitin regulates numerous cellular processes, including cell division, immune responses, and apoptosis. Ubiquitin-mediated control over these processes can be reversed by deubiquitinases (DUBs), which remove ubiquitin from target proteins and depolymerize polyubiquitin chains. Recently, much progress has been made in the DUBs. In humans, the ovarian tumor protease (OTU) subfamily of DUBs includes 16 members, most of which mediate cell signaling cascades. These OTUs show great variation in structure and function, which display a series of mechanistic features. In this review, we provide a comprehensive analysis of current progress in character, structure and function of OTUs, such as the substrate specificity and catalytic activity regulation. Then we discuss the relationship between some diseases and OTUs. Finally, we summarize the structure of viral OTUs and their function in immune escape and viral survival. Despite the challenges, OTUs might provide new therapeutic targets, due to their involvement in key regulatory processes.

关键词： ubiquitin     OTU deubiquitinases     structure     function     regulation    

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Heterologous expression of

Potter,Francis E. Nano

《环境科学与工程前沿（英文）》 2017年 第11卷 第2期 doi: 10.1007/s11783-017-0910-1

摘要： Maximum growth rate of mutant was 0.083 h with 5% CO . Maximum biomass concentration of mutant was 3.697 g·L . mutant can tolerate gas aeration with 15% CO . Maximum specific activity of laminarinase was 4.325 U·mg dry mass. Optimal pH and temperature of laminarinase activity were 8.0 and 70°C. The gene for the catalytic domain of thermostable endo-β-1,3-glucanase (laminarinase) LamA was cloned from Thermotoga maritima MSB8 and heterologously expressed in a bioengineered Synechococcus sp. PCC 7002. The mutant strain was cultured in a photobioreactor to assess biomass yield, recombinant laminarinase activity, and CO2 uptake. The maximum enzyme activity was observed at a pH of 8.0 and a temperature of 70°C. At a CO2 concentration of 5%, we obtained a maximum specific growth rate of 0.083 h , a biomass productivity of 0.42 g·L ·d , a biomass concentration of 3.697 g·L , and a specific enzyme activity of the mutant strain of 4.325 U·mg dry mass. All parameters decreased as CO2 concentration increased from 5% to 10% and further to 15% CO2, except enzyme activity, which increased from 5% to 10% CO2. However, the mutant culture still grew at 15% CO2 concentration, as reflected by the biomass productivity (0.26 g·L ·d ), biomass concentration (2.416 g·L ), and specific enzyme activity (3.247 U·mg dry mass).

关键词： Synechococcus sp. PCC 7002     Thermotoga maritima     LamA gene     Endo-β-1     3-glucanase     CO2 fixation    

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Sustainability of metal recovery from E-waste

Biswajit Debnath, Ranjana Chowdhury, Sadhan Kumar Ghosh

《环境科学与工程前沿（英文）》 2018年 第12卷 第6期 doi: 10.1007/s11783-018-1044-9

摘要：

Metal recovery techniques from electronic waste reported in literature.

Metal recovery processes followed in Industries from electronic waste.

Sustainability analysis of metal recovery processes from electronic waste.

关键词： E-waste     Metal recovery     Metal Recovery from E-waste (MREW)     Sustainability    

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标题 作者 时间 类型 操作

APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

期刊论文

Degradation of SARS-CoV-2 receptor ACE2 by the E3 ubiquitin ligase Skp2 in lung epithelial cells

Guizhen Wang, Qun Zhao, Hui Zhang, Fan Liang, Chen Zhang, Jun Wang, Zhenyin Chen, Ran Wu, Hong Yu, Beibei Sun, Hua Guo, Ruie Feng, Kaifeng Xu, Guangbiao Zhou

期刊论文

APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

期刊论文

Identification of an E3 ligase-encoding gene

Yanfei Zhang, Xinchun Zhao, Yongchun Zhou, Min Wang, Guangbiao Zhou

期刊论文

mTOR-targeted cancer therapy: great target but disappointing clinical outcomes, why?

Shi-Yong Sun

期刊论文

APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

Baohua CHU, Jia SUN, Huan DANG, Ziqing MA, Shuang ZHAO, Qingmei GUAN, Xuewei LI

期刊论文

Regulation of NLR stability in plant immunity

Tao WANG, Jiaxin LI, Qian-Hua SHEN

期刊论文

2020第四届环境与能源工程国际会议(IC3E 2020)

2020年03月12日

会议信息

基于S3C2410的GPRS数据终端的设计

孙学用

期刊论文

一种高效的异构车辆集群协同框架

Iftikhar AHMAD1,2,Rafidah Md NOOR2,Zaheed AHMED3,Umm-e-HABIBA3,Naveed AKRAM4, 5,Fausto Pedro GARCÍA MÁRQUEZ6

期刊论文

Genetic variants in the ADD1 and GNB3 genes and blood pressure response to potassium supplementation

Collaborative Research Group, De-Pei LIU PhD, Lai-Yuan WANG PhD, Jing CHEN MD, MSc, Jiang HE MD, PhD, Cashell E.RAO PhD, Charles GU PhD, James E. HIXSON PhD, Chung-Shiuan CHEN MS8, Paul K.

期刊论文

基于过滤—群聚注意力的低光增强递归网络

Fatma KHALLAF1,2, Walid EL-SHAFAI1,3, El-Sayed M. EL-RABAIE1, Naglaa F. SOLIMAN4, Fathi E.

期刊论文

The function and regulation of OTU deubiquitinases

Jiansen Du, Lin Fu, Yingli Sui, Lingqiang Zhang

期刊论文

Heterologous expression of

Potter,Francis E. Nano

期刊论文

Sustainability of metal recovery from E-waste

Biswajit Debnath, Ranjana Chowdhury, Sadhan Kumar Ghosh

期刊论文